Shared flowchart

Chemistry · L5 · Isotope Labeling and Tracer Studies · flowchart

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by @openstemUpdated Chemistry
Ester hydrolyzed in 18O-enriched waterWhich bond breaks: acyl-oxygen or alkyl-oxygen?18O label ends up in carboxylic acid product18O label ends up in alcohol productAnalyze product by mass spectrometry or NMRMechanism directly confirmed by label locationAlkyl-oxygen bond breaks: standard tetrahedral intermediate mechanismAcyl-oxygen bond breaks: alternative pathway

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