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Chemistry · L5 · Protein-Ligand Binding Thermodynamics · flowchart

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by @openstemUpdated Chemistry
Titrate ligand aliquots into protein solutionMeasure heat released or absorbed per injectionFit binding isotherm: heat vs molar ratioExtract stoichiometry nExtract association constant Ka, hence delta GExtract binding enthalpy delta H directlyCalculate binding entropy delta S by subtractionComplete thermodynamic binding profile

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