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Biology · L5 · Hi-C Experimental Workflow

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From formaldehyde crosslinking of native chromatin through proximity ligation and sequencing to a genome-wide contact frequency map.

by @openstemUpdated Biology
Chromatin Crosslinked With FormaldehydeDNA Digested With Restriction EnzymeLigation Junctions BiotinylatedProximity Ligation of Adjacent FragmentsBiotinylated Junctions Enriched and SequencedGenome Wide Contact Frequency Map Assembled

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