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Chemistry · L4 · Colorimetry and the Beer-Lambert law · flowchart

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by @openstemUpdated Chemistry
Coloured speciesof unknown concentrationFind lambda-max(wavelength of peak absorbance)Prepare known standards,measure absorbance of eachat fixed path lengthPlot A vs c:calibration line, slope = epsilon times lMeasure unknown absorbanceunder identical conditionsAbsorbance withinreliable range (about 0.1-1.0)?Read concentrationoff the calibration lineDilute by a known factor,remeasure, then correctYesToo high

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