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Chemistry · L5 · Chemical Biology and Activity-Based Probes · flowchart

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by @openstemUpdated Chemistry
ABP with small azide or alkyne handle added to live cellsWarhead reacts covalently with active enzyme onlyInactive enzyme copies remain unlabeledCells lysed after labeling is completeClick chemistry conjugates fluorophore or biotin reporter onto handleDetect labeled, formerly-active enzymes: gel fluorescence or streptavidin pulldownSignal reports which enzymes were catalytically active in the original live cell

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