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Biology · L5 · Prime Editing Reaction Sequence

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Step-by-step molecular sequence from pegRNA-directed nicking through reverse transcription to resolved edited DNA.

by @openstemUpdated Biology
Cas9 Nickase and pegRNA Bind Target DNANon-Complementary Strand NickedExposed 3 Prime End Anneals to Primer Binding SiteReverse Transcriptase Extends New Edited FlapEdited Flap Competes With Original 5 Prime FlapCellular Repair Resolves and Seals Edited Strand

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