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Chemistry · L5 · Interpreting a COSY Spectrum

Step-by-step workflow for reading a COSY spectrum: diagonal vs off-diagonal peaks, and how cross-peaks assemble into a full proton connectivity network.

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Acquire a COSY spectrum:¹H shift on both axesDiagonal peaks appear wherethe shift on axis 1 equalsthe shift on axis 2Off-diagonalcross-peak present?Those two protonsare not coupledThe two protons ARE coupledthrough 2 to 3 bondsTrace a line from thecross-peak to eachdiagonal positionRead off the pairof coupled shiftsRepeat for everycross-peak in the spectrumAssemble the full protonconnectivity networkNoYes

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