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Chemistry · L5 · Bioorthogonal Labeling Workflow with CuAAC

How azide and alkyne tags on two different molecules are brought together inside a living cell so the Cu(I)-catalyzed click reaction selectively links only the tagged partners, leaving the rest of the cell's chemistry undisturbed.

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Molecule of interest(e.g. a specific protein or sugar)Attach azide tag(metabolic or chemical labeling)Fluorescent dyeor biotin probeAttach alkyne tagIntroduce both taggedpartners into a living cellCu(I)-catalyzedclick reaction (CuAAC)Tagged molecule nowfluorescently labeledRest of the cell:untouchedSelective 1,4-triazole linkno reaction

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