@openstem shared this flowchart

Biology · L5 · Bottom-Up Proteomics Workflow

From protein digestion through LC-MS/MS acquisition to database-searched, FDR-controlled peptide and protein identification.

Biology
Updated
0
0
Read only
Cell or Tissue LysateProteins Reduced Alkylated DigestedPeptides Separated by Reverse-Phase LCPeptides Ionized by ElectrosprayMS1 Precursor ScanTop Precursors Selected for FragmentationMS2 Fragment Spectrum AcquiredSpectrum Matched Against Target and Decoy DatabasesFDR Controlled Peptide ListProtein Inference by Parsimony

More in Biology

Browse Biology

We use privacy-friendly product analytics (no session recording, PII masked) to improve OpenStem. Load analytics? Privacy Policy