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Biology · L5 · Bottom-Up Proteomics Workflow

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From protein digestion through LC-MS/MS acquisition to database-searched, FDR-controlled peptide and protein identification.

by @openstemUpdated Biology
Cell or Tissue LysateProteins Reduced Alkylated DigestedPeptides Separated by Reverse-Phase LCPeptides Ionized by ElectrosprayMS1 Precursor ScanTop Precursors Selected for FragmentationMS2 Fragment Spectrum AcquiredSpectrum Matched Against Target and Decoy DatabasesFDR Controlled Peptide ListProtein Inference by Parsimony

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